Product Description
BIOMIMESYS® Oncology is a new generation of mimetic hydroscaffold for 3D cell culture. The physicochemical properties (composition and architecture) of the ECM play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation. BIOMIMESYS® Oncology “Hydroscaffold” made of Hyaluronic acid (HA) and type I collagen crosslinked with Adipic acid dihydrazide (ADH), mimics the microenvironment of tumor cells thus creating the ideal condition for tumor cells to grow in 3D. Furthermore this scaffold provided cell binding sites with CD44 and RHAMM allowing cell-matrix and cell-cell interactions. Learn More
Key Features
- Provides binding sites for CD44 & RHAMM cell surface receptors
- Customizable
- Easy to use (ready to use 96 / 384 sterile plate)
- Compatible with all standard downstream analytical technologies
- Co-culture (tumor cells - normal cells - immune cells)
- Cancer cells proliferate and aggregate to form spheroid from the 5th day of culture
- Long term culture (at least 28 days)
- In vivo implantation possible (digestible hyaluronic acid)
- Ideal for high throughput screening (HTS) with 3D fluorescence imaging - Phenotypic Screening
Specifications
Composition:
• Hyaluronic acid (HA) (1.6MDa) – Provides binding sites for CD44 and RHAMM cell surface receptors
• Adipic acid dihydrazide (ADH) as crosslinker
• Collagen Type I
| Cell Lines | |
| Human brain metastasis | SA87 |
| Human breast adenocarcinoma | MCF-7 |
| Human breast carcinoma | CAL-51 / T-47D / MDA-MB231 / MCF10A / BT-549 |
| Human cervix adenocarcinoma | HeLa |
| Human colorectal adenocarcinoma | DLD-1 / HT29 / Caco-2 / HCT 116 |
| Human glioblastoma | CB109 / CB74 / CB191 /DIPG007 |
| Human liver hepatocellular carcinoma | HepG2 |
| Human liver hepatoma | PLC / PRF-5 |
| Human lung carcinoma | NCI-H460 |
| Human osteosarcoma | SaOs |
| Human ovarian carcinoma | IGROV-1 |
| Human pancreas carcinoma | PANC-1 |
| Human prostate cancer | PC3 |
| Normal human colon fibroblast | CCD18-co |
| Stem Cells | |
| Hematopoietic | CD34+ |
| Human induced pluripotent stem cells | hiPS |
1. Simon, T. et al. Direct Effect of Bevacizumab on Glioblastoma Cell Lines In Vitro. NeuroMolecular Medicine 16, 752–771 (2014). https://doi.org/10.1007/s12017-014-8324-8
2. Demange, E. et al. Survival of cord blood haematopoietic stem cells in a hyaluronan hydrogel for ex vivo biomimicry. J Tissue Eng Regen Med 7, 901–910 (2013). https://doi.org/10.1002/term.1482
3. Coquerel, B. et al. Elastin-derived peptides: matrikines critical for glioblastoma cell aggressiveness in a 3-D system. Glia 57, 1716–1726 (2009). https://doi.org/10.1002/glia.20884
4. David, L. et al. Collagens, stromal cell-derived factor-1alpha and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel. Cell Prolif 41, 348–364 (2008). https://doi.org/10.1111/j.1365-2184.2008.00515
5. David, L. et al. Hyaluronan hydrogel: An appropriate three-dimensional model for evaluation of anticancer drug sensitivity. Acta Biomaterialia 4, 256–263 (2008). . https://doi.org/10.1016/j.actbio.2007.08.012
6.David, L. et al. Reticulated hyaluronan hydrogels: a model for examining cancer cell invasion in 3D. Matrix Biology 23, 183–193 (2004). https://doi.org/10.1016/j.matbio.2004.05.005
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : HCS Pharma
|
Questions about this product? Email us at orders@iwai-chem.com or give us a call: (650) 486-1541 |
