iMatrix-411

Product Description

iMatrix-411 is a recombinant Laminin-411 E8 fragment protein used as a cell culture matrix. Laminin-411 is a major isoform that lines the basal membrane of the endothelial cells in capillary vessels and binds mainly to the cell surface transmembrane receptors integrin α6β1 and α71β1. The E8 fragment retains full binding activity toward integrin but lacks binding activities to other components. The switching of defined matrices is an important tool for guiding cells towards their desired fate. Studies have shown that changing the ECM from laminin-511 E8 fragment (iMatrix-511) to laminin-411 E8 during differentiation efficiently induces endothelial progenitor cells from iPS cells.  iMatrix-411 is highly purified human Laminin 411-E8 fragments recombinantly expressed by CHO-S cell.

Key Features

- Switching from laminin-511 E8 to laminin-411 E8 during differentiation induces endothelial progenitor cells
- Highly purified and refined human laminin-411 E8 fragment expressed by CHO-S cells
- Feeder-free cell culture

Application
iMatrix-411 can be used as cell culture matrix for various cell types including ES/iPS cells.

Specification
Product Size:
N-892041: 350 ug  (175 ug x 2 pcs.)
N-892042: 1050 ug (175 ug x 6 pcs.)
N-892043: 175 ug (175 ug x 1 pcs.) - SAMPLE SIZE **One time purchase only**

Format: Liquid Solution (175 ug/vial @0.5 mg/mL) Solvent: PBS(-)) 

Storage temperature: 2-15 °C 

Endothelial cells derived from human ES cell line KhES-1                  Endothelial cells derived from human iPS cell line 253G4

Documentation
Download Manual [PDF]
Download SDS [PDF]

Reference Literature
1. Takayama K et all., Laminin 411 and 511 promote the cholangiocyte differentiaion of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474 (1), 91-96, 2016. doi: 10.1016/j.bbrc.2016.04.075.
2. Ohta R et al., Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Scientific Reports, 6(35680),1-12, 2016. doi: 10.1038/srep35680.

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Nippi, Inc.