iMatrix-221

Product Description

iMatrix-221 is highly purified human Laminin 221-E8 fragments recombinantly expressed by CHO-S cell. Laminin-221 E8 fragment protein is known to bind to Intergin α7X2β1 of the cell surface, making an ideal cell culture matrix.

Application
iMatrix-221 is suitable for use for culture of various myoblast cell types, but most notably for cardiomyocytes.

Specification
Product Size : 350 ug  (175 ug x 2 pcs.)
Format: Liquid Solution (175 ug/vial @0.5 mg/mL) Solvent: PBS(-)) 
Storage temperature: 2-15 °C  

Documentation
Download Protocol [PDF]
Download SDS [PDF]

Reference Literature
1. Nishiuchi, R., Takagi, J., Hayashi, M., Ido, H., Yagi, Y., Sanzen N., Tsuji T., Yamada M., & Sekiguchi K. (2006). Ligand-binding specificities of laminin-binding integrins: A comprehensive survey of laminin–integrin interactions using recombinant α3β1, α6β1, α7β1 and α6β4 integrins. Matrix Biology, 25(3), 189-197. https://doi.org/10.1016/j.matbio.2005.12.001
2. Taniguchi, Y., Ido, H., Sanzen, N., Sato-Nishiuchi, R., Futaki, S., & Sekiguchi, K. (2009). The C-terminal Region of Laminin β Chains Modulates the Integrin Binding Affinities of Laminins. The Journal of Biological Chemistry, 284(12), 7820-31. https://doi.org/10.1074/jbc.M809332200
3. Israeli-Rosenberg, S., Manso, A. M., Okada, H., & Ross, R. (2014). Integrins and Integrin-Associated Proteins in the Cardiac Myocyte. Circular Research, 114(3), 572-586. https://doi.org/10.1161/CIRCRESAHA.114.301275
4. Method for producing cardiomyocyte population from pluripotent stem cells. Patent Publication No. WO2016043168 A1. 2017-6-22

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Nippi, Inc.